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This updated and expanded edition of a classic text allows novices and experienced researchers alike to apply both basic and sophisticated techniques of tissue culture. Coverage helps readers assess the role of cell cultures as models for in vivo processes, while expanded descriptions of protocols in areas of new technology and descriptions of improved serum-free media enables them to perform a wide range of specialized procedures without conducting additional research. New to this edition is coverage of induction of differentiation, the transformed phenotype, cytotoxicity and viability assays, culture of tumor tissue from animals and humans, and three-dimensional culture systems, including organotypic and histotypic cultures. Also includes a glossary, an international list of cell banks, an extensive listing of reagents and commercial suppliers, and over 600 literature references.
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